Photomanipulation
- High‐speed scanning: 20,000 points/second
- Simple auto‐calibration algorithm
- No optical switching
- Diffraction limited laser positioning
- 3-D targeting
- Streaming capabilities
- Visible light, UV, and IR light range
The iLas is an elegant tool for photomanipulation applications. By mounting on a microscope's epi‐fluorescence port, it can perform laser experments with no switching of the optical path. The iLas is ideal for 3-D targeting; an auto‐calibration algorithm makes it possible to accurately steer lasers even through deep and dense media. Integration with MetaMorph software can support multiple ROIs in pre-programmed or on‐the‐fly experiments.
FRAP
Fluorescence Recovery After Photobleaching (FRAP) is a powerful method for quantifying molecular diffusion or biological movement. The high resolution scanning of the iLas2 combined with it's fast speed and non-switching optical path make it an ideal solution for these experiments. Integration with MetaMorph software simplifies multidimension acquisition and data analysis. Lasers are available to match any fluorescent dye.
Ablation
Laser ablation is a potent technique for observing biological reactions and interactions. The iLas2 fully supports the high‐powered lasers necessary for these experiments. Standard solutions are available using 355 nm and 523 nm pulsed lasers, and custom solutions are possible for infrared and other custom experiments.
Photoactivation and photoconversion
Photoactivation and photoconversion are novel solutions for tracking the motion of proteins. Real-time control over laser power and position make the iLas2 is an ideal tool for this type of investigation. With complete microscope automation through MetaMorph, it is easy to combine photoactivation from the iLas with any other type of imaging.
Ablation
Laser ablation is a potent technique for observing biological reactions and interactions. The iLas2 fully supports the high‐powered lasers necessary for these experiments. Standard solutions are available using 355 nm and 523 nm pulsed lasers, and custom solutions are possible for infrared and other custom experiments.