VT-iSIM
Instantaneous super-resolution imaging system from VisiTech International
With VT-iSIM, the ground‐breaking high‐speed super resolution imaging system from VisiTech International, you can image at spatial and temporal resolutions never thought possible before.
With no requirement to use specific fluorophores and without any computation, the VT-iSIM produces super resolution images in real time at up to 1,000 frames per second.
This high temporal resolution is combined with a spatial resolution enhancement on all axes of up to 2× that of regular wide-field microscopy.
Images taken with VT-iSIM
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Monolayer of human fibroblasts infected with the parasite Toxoplasma gondii.
Human cells are not labeled.
Toxoplasma are expressing two FP tagged proteins.
EGFP-alpha-1-tubulin highlights the cortical microtubules, and an apical spot (the conoid) in both adult cells and in developing daughter cells (located inside the mother).
mCherryFP-APR1 highlights an apical ring, located immediately adjacent to the conoid, in both adult cells and in developing daughter cells.
Data courtesy of Dr. Ke Hu and Dr. Jacqueline Leung, Dept of Biology, Indiana University.
Microtubule growth from frog egg extract
Alexa-488 labelled porcine brain tubulin added to Xenopus laevis (frog) egg extract along with mCherry tagged EB1 protein to label the growing ends of microtubules.
Specimen from Akanksha Thawani, Sabine Petry lab, Princeton University
Specifications
| Spatial Resolution: | 125nm Laterally and 350nm Axially* |
| Temporal Resolution: | Scan Speed up to 1000fps, full frame |
| Pin Holes: | Selectable from 10-64um |
| Dichroic Changer: | Automated 3-Position Dichroic Changer |
| Emission Filter Changer: | Regular or High Speed 6-Position Emission Filter Changer |
| Excitation: | Up to 6 SS lasers from visible range (405, 445, 488, 514, 532, 561, 642) |
| FRAP: | Fully integrated FRAP add-on available |
| Bright Field by-pass: | BF by-pass mode available for regular wide field imaging |
| Sync: | Perfect camera & excitation sync comes as standard |
| Camera Specification: | For accurate sampling camera must have pixel size less than 6.5 um |
| Microscope Specification: | Nikon, Olympus, Zeiss, Leica. Connection via regular C-mount |
| Software: | MetaMorph, Micro-Manager, VisiTech International’s VoxCell Scan acquisition software |
* Spatial resolution measured using PSFj with Nikon 100x 1.45NA Lens